Composite

Part:BBa_M36085

Designed by: Ashley Hammerbacher Celina Malavé   Group: Stanford BIOE44 - S11   (2014-04-30)


Winter Flounder Antifreeze Protein Actuator With Secretion Tag

This is our actuator to generate antifreeze protein activity in yeast. We are expressing the winter flounder AFP to be able to minimize ice growth. It includes the yeast secretion tag so that it is expressed outside of the cell and so that it will be expressed in the solution.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1
    Illegal BamHI site found at 102
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 123
    Illegal NgoMIV site found at 141
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None